The BA amplifiers are wide-bandwidth high impedance amplifier designed for intracellular current clamp recordings with sharp microelectrodes, in perforated or tight seal patch configurations as well as for electroporation and iontophoresis. Currently, npi BA amplifiers are used in vertebrate (rat, mouse, cat, fish etc.) and invertebrate (leech, crab, snail, insect etc.) neurons, muscle cells, other excitable tissue and plant cells.
Each amplifier of the BA series is housed in a 19″ rackmount cabinet and equipped with functions like two ranges for bridge balance, an oscillation shut-off circuit for protecting the preparation, two potential and two current ranges. The amplifier comes with a small headstage with a holding bar (or a mounting plate / dovetail adapter on request) and a BNC connector for the electrode holder, and can be attached directly to a micromanipulator.
The BA-01X is the standard bridge amplifier fot intracellular current clamp recordings. Having an extended current range (x10, i.e. +/. 120 nA max.) this amplifer is also suitable for electroporation of single cells for non-invasive, juxtacellular filling of cells with dyes or plasmids. The BA-03X has additionally high and low pass filters for the recorded potenial, and a potential gain up to 1000. Therefore, it allows extracellular recordings as well.
A bridge amplifier is also available as module for the EPMS-07 system.
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HEADSTAGE
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Input voltage range: ±1000 mV
Operating voltage: ±15 V Enclosure: Size: 23 x 70 x 26 mm, grounded Holding bar: Size: length 150 mm, diameter 8 mm Electrode connector: BNC connector with driven shield Ground connector: 2.4 mm connector Input resistance: >1013 Ω Current range x1: ±12 nA into 100 MΩ Current range x10: ±120 nA into 100 MΩ |
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ELECTRODE PARAMETER CONTROLS
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BIAS: ±150 pA in range x1 and x10, adjustable with ten-turn control
OFFSET: ten-turn control, range ±200 mV CAPACITY COMPENSATION: range 0-30 pF, ten-turn control. |
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CELL PENETRATION
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Overcompensation of capacity compensation (BUZZ), duration ~2 – ~50 ms (slightly dependent on the setting of capacitance compensation), timer controlled with linear control,
accessible with remote switch |
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ELECTRODE CLEAR CIRCUIT
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Application of max. DC currents (-Imax or +Imax),
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OSCILLATION SHUT-OFF
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Turns off current injection and capacity compensation, function indicated by red / green LED,
disabled / off / reset switch, threshold set with linear control (0-1200 mV). |
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BRIDGE BALANCE
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adjustable with ten-turn control, 0-100 MΩ and 0-1000 MΩ with toggle switch.
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ELECTRODE RESISTANCE test
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10 mV / MΩ obtained by application of square current pulses ±1 nA, display XXXX MΩ.
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BANDWIDTH and SPEED of RESPONSE
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Full power bandwidth (Re = 0): >30 kHz, rise time (10 % – 90 %) <10 µs (Re = 100 MΩ),
<5 µs (Re = 5 MΩ). |
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OUTPUTS (BA-01X)
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Impedance: 50 Ω;
Current x1: sensitivity 0.1 V / nA, display XX.XX nA, resolution 10 pA; Current x10: sensitivity 0.1V / nA, display XXX.X nA, resolution 100 pA; Potential: BNC connector, sensitivity x10 mV or x100 mV, selectable with toggle switch, display XXXX mV. |
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OUTPUTS (BA-03X)
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Impedance: 50 Ω;
Current range x1: sensitivity 1 V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XX.XX nA, resolution 10 pA; Current range x10: sensitivity 0.1V / nA to 10 V / nA, selectable by rotary switch, (7 steps); display XXX.X nA, resolution 100 pA; Potential: BNC connector, sensitivity x10 mV to x1000 mV, selectable with rotary switch (7 steps), display XXXX mV. |
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CURRENT INPUTS RANGE x1
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0.1 nA / V, 1 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 10 pA, polarity selectable with toggle switch,
holding current range ±10 nA with ten-turn control. |
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CURRENT INPUTS RANGE x10
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1 nA / V, 10 nA / V, inputs equipped with on / off toggle switch, gating input with digital control of current step size and a resolution of 100 pA, polarity selectable with toggle switch,
holding current range ±100 nA with ten-turn control. |
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POWER REQUIREMENTS
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115 / 230 V AC, 25 W
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DIMENSIONS
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19″ rackmount cabinet, 19″ (483 mm) wide, 10″ (250 mm) deep, 3.5″ (88 mm) high |
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